Search results for "deficient diet"

showing 3 items of 3 documents

Decreasing dietary linoleic acid promotes long chain omega-3 fatty acid incorporation into rat retina and modifies gene expression

2011

International audience; Age-related macular degeneration (AMD) may be partially prevented by dietary habits privileging the consumption of ω3 long chain polyunsaturated fatty acids (ω3s) while lowering linoleic acid (LA) intake. The present study aimed to document whether following these epidemiological guidelines would enrich the neurosensory retina and RPE with ω3s and modulate gene expression in the neurosensory retina. Rat progenitors and pups were fed with diets containing low or high LA, and low or high ω3s. After scotopic single flash and 8-Hz-Flicker electroretinography, rat pups were euthanized at adulthood. The fatty acid profile of the neurosensory retina, RPE, liver, adipose tis…

CD36 AntigensMaleMESH : RNA MessengerMESH: 5-Lipoxygenase-Activating ProteinsMESH : Receptors LDLMESH: Electroretinography0302 clinical medicineMESH: Fatty Acids Omega-3MESH: AnimalsMESH : Retinal Ganglion Cellschemistry.chemical_classification0303 health sciencesMESH : Gene Expression RegulationMESH : ElectroretinographyMESH: RetinaMESH: Chromatography GasMESH: Dietary Fats Unsaturateddocosahexaenoic acidpolyunsaturated fatty acidSensory Systems3. Good healthnutritionMESH: Photic StimulationAdipose TissueMESH: Adipose Tissuemedicine.medical_specialtyChromatography Gasmacular degenerationLinoleic acidMESH : Arachidonate 12-LipoxygenaseArachidonate 12-LipoxygenaseMESH : Adipose TissueMESH: Arachidonate 12-Lipoxygenasepufa03 medical and health sciencesMESH : Dietary Fats UnsaturatedlipidElectroretinographyRats Long-EvansRNA MessengerMESH: Linoleic AcidMESH: Antigens CD36MESH : RetinaFatty acidMESH: Retinal Ganglion Cellseye diseasesOphthalmologyEndocrinologychemistryMESH: Receptors LDL030221 ophthalmology & optometryATP-Binding Cassette Transportersn 3MESH: FemalePhotic StimulationMESH: LiverRetinal Ganglion CellsretinaMESH : 5-Lipoxygenase-Activating Proteinsgenetic structures[ SDV.AEN ] Life Sciences [q-bio]/Food and Nutritionretinal pigment epitheliumelectroretinogramMESH : Photic StimulationAdipose tissueangiogenesischemistry.chemical_compoundMESH : FemaleMESH : Rats Long-Evans2. Zero hungermedicine.diagnostic_testMESH : RatsMESH: Real-Time Polymerase Chain ReactionMESH: Gene Expression RegulationMESH : Antigens CD36medicine.anatomical_structureLiverALOX12BiochemistryMESH: ATP-Binding Cassette TransportersFemaleATP Binding Cassette Transporter 1Polyunsaturated fatty acidMESH : Fatty Acids Omega-3MESH: RatsbrainMESH : Male5-Lipoxygenase-Activating ProteinsMESH : Real-Time Polymerase Chain Reactionrhesus monkeyBiologyReal-Time Polymerase Chain ReactionMESH : Chromatography GasLinoleic AcidCellular and Molecular NeuroscienceDietary Fats UnsaturatedMESH : Linoleic AcidMESH: Rats Long-EvansInternal medicineFatty Acids Omega-3medicineAnimalsMESH : ATP-Binding Cassette TransportersOmega 3 fatty acidMESH: RNA Messenger030304 developmental biologydeficient dietRetinal pigment epitheliumMESH : LiverMESH: MaleRatsGene Expression RegulationReceptors LDLgene expressionMESH : Animalssense organs[SDV.AEN]Life Sciences [q-bio]/Food and NutritionElectroretinographyExperimental Eye Research
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Mboat7 down-regulation by hyper-insulinemia induces fat accumulation in hepatocytes.

2020

Background: Naturally occurring variation in Membrane-bound O-acyltransferase domain-containing 7 (MBOAT7), encoding for an enzyme involved in phosphatidylinositol acyl-chain remodelling, has been associated with fatty liver and hepatic disorders. Here, we examined the relationship between hepatic Mboat7 down-regulation and fat accumulation. Methods: Hepatic MBOAT7 expression was surveyed in 119 obese individuals and in experimental models. MBOAT7 was acutely silenced by antisense oligonucleotides in C57Bl/6 mice, and by CRISPR/Cas9 in HepG2 hepatocytes. Findings: In obese individuals, hepatic MBOAT7 mRNA decreased from normal liver to steatohepatitis, independently of diabetes, inflammatio…

Research paperTGFβ Transforming Growth Factor BetaIntracellular SpaceCRISPR Clustered Regularly Interspaced Short Palindromic RepeatshHEPS Human HepatocytesMice0302 clinical medicineLPIAT1DAG Diacylglyceroli.p. Intraperitonealmedia_commonFatty AcidsGeneral Medicine3. Good health030220 oncology & carcinogenesisHOMA-IR homeostasis Model Assessment of Insulin ResistanceMPO morpholinolcsh:Medicine (General)medicine.medical_specialtyPE Phosphatidyl-EthanolamineNashGeneral Biochemistry Genetics and Molecular Biology03 medical and health sciencesTNFα tumor Necrosis Factor AlphaLDL Low Density LipoproteinsHyperinsulinismNAFLDSD Standard Dietmedia_common.cataloged_instanceHumansCPT1 Carnitine Palmitoyltransferase IPhosphatidylinositolGene SilencingEuropean unionVLDL Very Low Density Lipoproteinlcsh:RhHSC Human Hepatic Stellate Cellsmedicine.diseaseLipid MetabolismOA Oleic AcidCI Confidence IntervalMboat7 Membrane bound O-acyltransferase domain containing 7MCD methionine choline deficient diet030104 developmental biologyEndocrinologychemistryCDP Cytidine-DiphosphateFOXO1 Forkhead Box protein O1NAFLD nonalcoholic fatty liver diseaseSteatohepatitisBMI Body Mass IndexCL CardiolipinAcyltransferases0301 basic medicineAlcoholic liver diseaseCXCL10 C-X-C Motif Chemokine 10lcsh:Medicinechemistry.chemical_compoundNon-alcoholic Fatty Liver DiseaseIFG Impaired Fasting GlucoseAPOB Apolipoprotein BNonalcoholic fatty liver diseasePIP Phosphatidyl-Inositol-PhosphateSteatohepatitisqRT-PCR quantitative Real Time Polymerase Chain ReactionMice Knockoutlcsh:R5-920ORO Oil Red O StainingPI PhosphatidylinositolFatty liverTM6SF2 Transmembrane 6 Superfamily Member 2PhospholipidTAG TriglyceridesNASH Nonalcoholic SteatohepatitisLipogenesisLPA Lyso-Phosphatidic AcidPhosphatidylinositolSignal TransductionPS Phosphatidyl-SerinePA Palmitic AcidALD alcoholic liver diseasePC Phosphatidylcholinei.v. IntravenousFATP1 Fatty Acid Transport Protein 1Models BiologicalInternal medicinemedicineAnimalsNonalcoholic fatty liver diseasePPARα Peroxisome Proliferator-Activated Receptor alphaObesityG3P Glyceraldehyde-3-PhosphateSREBP1c Sterol Regulatory Element-Binding Protein 1HDL High Density Lipoproteinsbusiness.industryPI3K Phosphatidylinositol 3 KinaseMembrane ProteinsNHEJ Non-Homologues End JoiningPNPLA3 Patatin-like Phospholipase Domain-containing-3MTTP Microsomal Triglyceride Transfer ProteinLPIAT1 Lysophosphatidylinositol Acyltransferase 1TMC4 Transmembrane Channel-Like 4Disease Models AnimalGene Expression RegulationHepatocytesFOXA2 Forkhead Box A2mTOR mammalian target of RapamycinSteatosisInsulin ResistancebusinessPG Phosphatidyl-GlycerolFABP1 Fatty Acid-Binding Protein 1 FAS Fatty Acid SynthaseT2DM Type 2 Diabetes MellitusEBioMedicine
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Investigating fibrosis and inflammation in an ex vivo NASH murine model.

2020

Nonalcoholic fatty liver disease (NAFLD) is the most common liver disease, characterized by excess fat accumulation (steatosis). Nonalcoholic steatohepatitis (NASH) develops in 15–20% of NAFLD patients and frequently progresses to liver fibrosis and cirrhosis. We aimed to develop an ex vivo model of inflammation and fibrosis in steatotic murine precision-cut liver slices (PCLS). NASH was induced in C57Bl/6 mice on an amylin and choline-deficient l-amino acid-defined (CDAA) diet. PCLS were prepared from steatohepatitic (sPCLS) and control (cPCLS) livers and cultured for 48 h with LPS, TGFβ1, or elafibranor. Additionally, C57Bl/6 mice were placed on CDAA diet for 12 wk to receive elafibranor…

0301 basic medicineLipopolysaccharidesLiver CirrhosisMalePhysiologyHEPATOCYTESLiver diseaseMice0302 clinical medicineChalconesFibrosisNon-alcoholic Fatty Liver DiseaseNonalcoholic fatty liver diseaseCells CulturedINSULIN-RESISTANCEGastroenterologyElafibranorTGF-BETALiver030211 gastroenterology & hepatologyCHOLINE-DEFICIENT DIETEXPRESSIONmedicine.medical_specialtyEARLY-ONSETIn Vitro TechniquesCollagen Type IProinflammatory cytokineTransforming Growth Factor beta103 medical and health sciencesIn vivoPhysiology (medical)Internal medicinemedicineAnimalsHEPATIC STEATOSISFATTY LIVER-DISEASEInflammationPRECISION-CUT LIVERHepatologybusiness.industrymedicine.diseaseLipid MetabolismDietMice Inbred C57BLDisease Models Animal030104 developmental biologyEndocrinologyPROLIFERATOR-ACTIVATED RECEPTORSSteatosisPropionatesbusinessTranscriptomeEx vivoAmerican journal of physiology. Gastrointestinal and liver physiology
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